Tag: Reader Questions

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Feeding Ant Queens

Mike wrote to the “Consult-Ant” with a number of questions about ants. I am going to try to answer each one in a separate post. For the original list of questions and links to all answers, visit here.

Question 8. If I were to catch a queen ant, would it be recommended to feed her some protein as well as sugary foods like honey? I was thinking about this because I’ve read that the eggs and larvae would need protein to grow properly (I’m guessing for muscle development and such). So is peanut butter a good protein choice, in substitute of other insects? I’m worried that peanut butter wouldn’t have all the essential amino acids because peanut butter is an incomplete protein. But I’m not sure about ant development and physiology, so I’m curious.

The answer to this question depends on what kind of ant queen you find. In many temperate ant species, the queen starts a colony via what is called “claustral” founding. In this case, she seals herself off from the world as much as possible, often forming a special chamber. Then the queen lays her first batch of eggs. Until those eggs complete development and eclose into worker ants, it is likely the queen will not feed at all. Usually a queen in this case is surviving on the fat reserves she stored while in her natal colony, as well as the breakdown products from the deterioration of her flight muscles. The queen has no further use for her flight muscles once she has removed her wings, so the muscles break down.

queen-1
Carpenter ant queens have claustral founding.

You might want to give new queens a bit of honey- or sugar-water solution absorbed into a piece of cotton ball or paper towel wad. Just so you know, adding any food always increases the chance of introducing unwanted molds or bacteria.

Once the little colony is underway, you can start feeding. To figure out the best foods, do some research on your particular species. Many scientists prepare a general diet useful for a variety of species. One of the earliest is known as the Bhatkar diet, named for the scientist who developed it. Hoelldobler and Wilson have a section on culturing ants in the back of their book, The Ants. It gives the recipe for Bhatkar diet. (Try Google Books). The Myrmecology Forum also has a link to a .pdf file of the original paper with the recipe. Basically it is honey, an egg, vitamins and agar (available online). You mix it up, cook it to get the agar to thicken, and then cool.

Supplement the diet of predator or scavenger ants with small amounts of freshly killed insects, such as mealworms or crickets, both available at many pet stores. You can also maintain mealworm/cricket cultures of your own. I have used tuna in a pinch. I’ve never tried peanut butter. It might be a bit sticky and the ants could get stuck in it, depending again on what kind of ants you have.

The maker of this video has some good ideas, such as putting the food on a bit of foil, to help with clean up. I would also soak it into cotton or paper towel to prevent workers getting stuck and drowning, but this person didn’t find that necessary.

For more information:

BHATKAR, A. P., AND W. H. WHITCOMB. (1970). Artificial diet for rearing various species of ants. Florida Entomol. 53: 229-232.

COHEN, A. C., AND L. K. SMITH. (1998). A new concept in artificial diets for Chrysoperla rufilabris: The efficacy of solid diets. Biol. Control. 13: 49-54. This diet has been used for fire ants successfully.

Ant Cam website has many FAQ’s useful for ant farms. Try What is good food for ants in my ant farm? for two different diets and advice.

Does anyone have any other great websites or papers with useful information on feeding queen ants and new colonies?

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Ant Trophallaxis

Mike wrote to the “Consult-Ant” with a number of questions about ants. I am going to try to answer each one in a separate post. For the original list of questions and links to all answers, visit here.

7) I’ve read a little about trophallaxis (I hope I spelled that right, because Microsoft Word does not help in scientific terminology), but I don’t see ants perform trophallaxis much. I was observing pavement ants though, so perhaps it has to do with the species. But my question is, do they perform it more underground, as in their colony? Or is it all because these ants weren’t hungry at the time? And what does an ant have to do induce another ant to perform trophallaxis? Some kind of touch communication by antennae?

Yes, you did spell trophallaxis correctly.

trophallaxis

When ants feed on liquid food, it goes to the crop, an organ inside the gaster. The crop can be called the ant’s “social stomach” because food collected there may be given to other ants via regurgitation. Ants pass food from mouth to mouth via a process called trophallaxis, which may look like kissing to the untrained observer. Any food the ant doesn’t share with other ants is passed to the ant’s own midgut where it is digested.

Most of the ants you see at the surface are foraging ants intent on getting food back to the nest as soon as possible. Outside the nest is a dangerous place. Thus, it is more common for the ants to engage in trophallaxis underground, as you surmised.

Dr. Bert Hoelldobler studied how certain beetles are able to trick ants into giving them food this way. He was able to show that tapping an ant lightly with the antennae and/or forelegs (tarsi) in the right parts of the body, usually the labium, is enough to trigger an ant to regurgitate food. He was then able to use a human hair to touch an ant and get it to regurgitate.

Dr. Alex Wild from Myrmecos blog, has an absolutely fabulous video of ants performing trophallaxis, which due to the wonders of YouTube, I can show you here. Thanks Dr. Wild! He also has some awesome photos of trophallaxis.

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Memory in Ants

Mike wrote to the “Consult-Ant” with a number of questions about ants. I am going to try to answer each one in a separate post. For the original list of questions and links to all answers, visit here.

6) So ants have pheromones they lay to provide trails to sources of food, but I’ve read somewhere that ants have memory. Can you explain more about that? A ‘leader’ ant would teach a ‘follower’ ant towards a food source. Is that true? with only certain species?

Although their brains are obviously very different from ours, we do have some evidence that worker ants do have memories. For example, James Hare and Thomas Eisner showed that workers ants that had been exposed to moth eggs (Utetheisa ornatrix) full of nasty alkaloids would still avoid moth eggs days later. But not just avoid toxic eggs. In an elegant study, Hare and Eisner were able to rear moths that did not contain the alkaloids, producing eggs that were perfectly edible. Ants with no previous exposure to the alkaloid-laden eggs would eat the alkaloid-free ones readily. It was the workers with previous experience (up to 33 days prior!) that would avoid both kinds of moth eggs.

In a paper in Myrmecological News, Dornhous and Franks give an overview of cognition in ants, and other insects. They report that foraging ants use memory to find food sources, and can even remember times of day the food sources are available. Ants can also remember where they have looked for new nests. Wood ants apparently memorize visual “snapshots” of landmarks as they travel about. Although chemical cues are important, if an ant is dropped into its environment in a place with no chemical trails, it might be able to orient back to the nest using visual cues. Ants have been known to be able to learn to move through mazes since Schneirla’s work in the early 1940’s, so are able to improve their performance of certain tasks.

Ant running a maze.

Dornhous and Franks point out that most of the studies of cognition in insects have been carried out with honey bees and fruit flies, for various reasons. There is still a lot of work to be done on ants.

As for the leader and follower ants, would you believe that ants could be teachers? When researchers looked at how ants lead their nest mates to new sources of food or a better nest, they found that the experienced ants actually taught the others where to find the target during a process known as “tandem-running.” Tandem running is when one ant follows another closely while running.

To discover whether or not the ants were actually teaching each other, first the investigators needed a solid definition of what it means to be a teacher. They decided that to be a true teacher, the ant must change its behavior when it encounters an inexperienced ant. At a cost to its own ability to perform the task, it must set an example so that the untrained ant can learn more quickly than it could have without training.

Sure enough, teacher ants approached uninformed nest mates and literally showed them the way to the food or new nest by running ahead. The follower gives feedback to the leader by continuous touching with her antennae. By teaching others the path to take, the teacher ant ran slower than it would have without a follower, but the pupil ant found the target in two-thirds the time it would have taken without help.

To learn more:

Dornhous, A. and N. R. Franks. (2008). Individual and collective cognition in ants and other insects (Hymenoptera:  Formicidae). Myrmecological News. 11:  215-226.

Franks, N.R. and T. Richardson. (2006). Teaching in tandem-running ants. Nature. 439:  153.

Hare, J.F. and T. Eisner. (1993). Pyrrolizidine alkaloid deters ant predators of Utetheisia ornatrix eggs: effects of the alkaloid concentation, oxidation state, and prior exposure of ants to alkaloid-laden prey. Oecologia. 96: 9-18.

R. Josens, C. Eschbach, and M. Giurfa. (2009). Differential conditioning and long-term olfactory memory in individual Camponotus fellah ants. J. Exp. Biol. 212:1904-1911 Retrieved from http://jeb.biologists.org/cgi/content/abstract/212/12/1904

Nowbahari, E. (2007). Learning of colonial odor in the ant Cataglyphis niger (Hymenoptera; Formicidae). Learning & Behavior, 35: 87-94. Retrieved from http://lb.psychonomic-journals.org/content/35/2/87.abstract ( free .pdf available)

Y. Provecho and R. Josens. (2009).Olfactory memory established during trophallaxis affects food search behaviour in ants. Journal of Experimental Biology. 212: 3221-3227. Retrieved from http://jeb.biologists.org/cgi/content/abstract/212/20/3221 (.pdf available)

Schneirla, T.C. (1943). The nature of ant learning II. The intermediate stage of segmental maze adjustment. Journal of Comparative Psychology. 35:  149-176.

What is she thinking?
What is she thinking?
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Questions About Ant Pheromones

Mike wrote to the “Consult-Ant” with a number of questions about ants. I am going to try to answer each one in a separate post. For the original list of questions and links to all answers, visit here.

4)      Besides making trails and setting alarms, are there any other kinds of unique pheromones? Such as, attracting the queen ant or inducing certain behaviors like digging.

Sure, there are a lot of different pheromones in ants. Ants use pheromones to

  • recruit to food sources,
  • mark the way to new nest sites during emigration
  • aggregate
  • mark territories
  • recognize nestmates
  • “call”- the release of pheromones by reproductive females to attract males
  • induce nestmates to defend the nest (alarm)

to name a few. Some queens release pheromones that induce workers to kill larval forms that would become reproductives, or that prevent virgin queens from shedding their wings. In carpenter ants, the males release a pheromone from their mandibular glands that signals to the female reproductives that it is time to fly from the nest and join the mating swarm. (Holldobler and Maschwitz 1965, as cited in LD Hansen and JH Klotz, Carpenter Ants of The United States and Canada).

Behaviors like digging can also be stimulated by non-chemical signals, such as stridulation (making sounds) by buried worker ants trying to get nestmates to dig them out.

Knowledge of ant pheromones a bit rusty?  Let me explain what we are talking about. Ants produce chemicals, in fact they are walking chemical factories. Bert Holldobler and E.O. Wilson wrote extensively about how ants use chemicals for communication in Chapter 7 of their book, The Ants (starting on page 227).

The term “pheromone” is defined as a substance released by an organism to the outside that causes a specific behavioral or physiological reaction in a receiving organism of the same species. [Nordlund, D. A. and W. J. Lewis. (1976). Terminology of chemical releasing stimuli in intraspecific and interspecific interactions. J. Chem. Ecol. 2: 211-220.]

For example, this video shows an ant laying trail pheromones. Other ants will detect the chemical in the trail and follow it back to the food.


All of these chemicals are made in exocrine glands found throughout the ant’s body.

In the head are the

  • mandibular gland – often produces alarm and defense compounds, extends all the way to the gaster in certain Camponotus
  • maxillary gland -source of digestive enzymes
  • propharyngeal gland – source of digestive enzymes
  • postpharyngeal gland – source of cuticular hydrocarbons (colony odor) and also food for larvae
  • antennal glands – found in Solenopsis fire ants

In the alitrunk are the

  • labial gland – equated with a salivary gland
  • metapleural gland (labeled in illustration) – source of antibiotic compounds, occasionally alarm pheromones/repellents

    • (Archetype has an awesome post about the structure and function of the metapleural gland.)

In the gaster, we find the

  • poison gland – source of defensive formic acid in Formicinae, recruitment to food in some myrmecines
  • rectal gland
  • sternal gland (Pavan’s gland) – trail pheromones
  • Dufour’s gland – often the source of trail pheromones
  • pygidial gland
  • etc.

ant-glands

The presence or absence of these glands, their structure and their contents varies between ant species, and even within individual caste members of a given species. The pheromones used and the message they contain are species specific by definition. That means what scientists learn about the pheromones of one species may not have general application to any other species, although some have been found to overlap.

To learn more about the chemistry of ant pheromones, try The Pherobase, a website of known pheromones, attractants, etc. You can search the database by animal taxon, or go directly to the Formicidae page.

There you can find out, for example, that the trail pheromone for Atta texana is me-4me-pyrrole-2-carboxylate, and what it’s structure looks like. Or the mandibular gland components of the exploding carpenter ants of Camponotis cylindricus group. Solenopsis invicta queens apparently produce (E)-6-(1-Pentenyl)-2-pyranone for recognition, that is so that the workers know she is their queen. This site is really cool if you are an ant geek.

5)      The ant uses their antennae to pick up ant pheromones, so if that’s the case, then do ants necessarily ‘smell’ food if the pheromone is blown towards the ant’s way? Essentially speaking, can ants smell their way to food?

Oh, definitely yes. In a recent post I discussed how certain ants can even use odors as a type of chemical map to find their way around. Their way of orienting can be called a “topochemical” map. Of course ants may use a variety of cues to find food, including sight, but it makes sense that they can detect plant chemicals and even those of sources of honeydew like aphids and scales.

If anyone has posts or references that might be helpful to Mike, please let us know.